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8225
Detection of Pathogenic Escherichia coli Strains Pollution in Red Deer Meat in Latvia and Determination the Compatibility of VT1, VT2, eae A Genes in their Isolate
Abstract:
Tasks of the work were study the possible E.coli contamination in red deer meat, identify pathogenic strains from isolated E.coli, determine their incidence in red deer meat and determine the presence of VT1, VT2 and eaeA genes for the pathogenic E.coli. 8 (10%) samples were randomly selected from 80 analysed isolates of E.coli and PCR reaction was performed on them. PCR was done both on initial materials – samples of red deer meat - and for already isolated liqueurs. Two of analysed venison samples contain verotoxin-producing strains of E. coli. It means that this meat is not safe to consumer. It was proven by the sequestration reaction of E. coli and by comparison of the obtained results with the database of microorganism genome available on the internet that the isolated culture corresponds to region 16S rDNS of E. coli thus presenting correctness of the microbiological methods.
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[1] L. W. Riley, R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells, B. R. Davis, R. J. Herbert, E. S. Olcott, L. M. Johnson, N.T. Hargrett, P. A. Blake, and M. L. Cohen, Haemorrhagic colitis associated with an Escherichia coli serotype New England Journal of Medicine, vol. 308, 24, March. 1983, pp. 681-685.
[2] H. Karch, P. I. Tarr and M. Bielaszewska, Enterohaemorrhagic Escherichia coli in human medicine International Journal of Medical Microbiology, vol. 295, Isuue 6-7, Oct. 2005, pp. 405 -418.
[3] Ch. Bindu Kiranmayi, N. Krishnaiah, and E. Naga Mallika Escherichia coli O157:H7 - an emerging pathogen in foods of animal origin. Veterinary World, vol. 3, No. 8, Aug. 2010, pp. 382-389.
[4] A. V. Tutenel, D. Pierard, J. Van Hoof, M. Cornelis, and L. De Zutter, ÔÇ×Isolation and molecular characterization of Escherichia coli O157 isolated from cattle, pigs and chickens at slaughter International Journal of Food Microbiology, vol. 84, issue 1, 15, July. 2003, pp. 63-69
[5] M. A. Karmali, V. Gannon and J. M. Sargeant Verocytotoxin producing Escherichia coli (VTEC). Veterinary Microbiology 140, 2010, pp. 360- 370.
[6] N. A. Strockbine, J. G. Wells, C. A. Bopp, and T. J. Barrett, "Overview of detection and subtyping methods, in Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. Coli Strains, J.B Kaper., A.D. O-Brien.Ed. Washington, American Society for Microbiology Press, 1998, pp. 331-356.
[7] M. Blanco, J. E. Blanco, A. Mora, G. Dahbi, M. P. Alonso, E. A. Gonzalez, M. I. Bernardez, and J. Blanco, Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin) - producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene Journal of Clinical Microbiology 42, 2004, pp. 645-651.
[8] J. C. Paton, and A. W. Paton, Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clinical Microbiology Reviews, vol.11, issue 3, July. 1998, pp. 450-479.
[9] E. Lahti, M. Keskimaki, L. Rantala, P. Hyvonen, A. Siitonen and T. Honkanen-Buzalski Occurrence of Escherichia coli O157 in Finish cattle. Veterinary Microbiology, vol. 79, issue 3, Apr. 2001, pp. 239- 251.
[10] A. García-Sánchez, S. Sánchez, R. Rubio, G. Pereira, J.M. Alonso, J. Hermoso de Mendoza, and J. Rey Presence of Shiga toxin-producing E.coli 157:H7 in a survey of wild artiodactyls. Veterinary Microbiology, vol.121, issue 3/4, Apr. 2007, pp. 373-377.
[11] J. M. Sargeant, D. J. Hafer, J. R. Gillespie, R. D. Oberst, and S.J. Flood Prevalence of Escherichia coli O157:H7 in white-tailed deer sharing rangeland with cattle. Journal of the American Veterinary Medical Association, vol. 215, No.5, 1999, pp. 792-794.
[12] J. W. Yoon, and C. J. Hovde, "All blood, no stool: enterohemorrhagic Escherichia coli O157:H7 infection Journal Veterinary Science 9, 2008, pp. 219-231
[13] T. Rabatsky-Ehr, D. Dingman, R. Marcus, R. Howard, R. Kinney, and P. Mshar. Deer meat as the source for a sporadic case of Escherichia coli O157:H7 infection. Emerging Infectious Diseases, 8 (5), 2002, pp. 525- 527.
[14] A. Miko, K. Pries, S. Haby, K. Steege, N. Albrecht, G. Krause, and L. Beutin. Assessment of Shiga toxin-producing Escherichia coli isolates from wildlife meat as potential pathogens for humans. Applied and Environmental. Microbiology. 75, 2009, pp. 6462-6470.
[15] S. Lehmann, M. Timm, H. Steinruck, and P. Gallien. Nachweis von STEC im Kot von Hochwild und in Wildfleischwirtschaft. Fleischwirtschaft, vol. 86, No. 4, 2006, s. 93 -96.
[16] M. Louie, J. C. S. De Azavedo, M.Y. Handelsman, C. G. Clark, B. Ally, M. Dytoc, P. Sherman, J. Brunton. Expression and Characterization of the eaeA Gene Product of Escherichia coli Serotype 0157:H7. Infection and Immunity, vol.61, No.10, Oct. 1993, pp. 4085-4092.
[17] R. A. Hazarika, D. K. Singh, K. N. Kapoor, R. K. Agarwal, A. B. Pandey, and D.N. Rajkumar. Detection and characterization of verotoxin-producing Escherichia coli (VTEC) isolated from buffalo meat. Journal of Food Safety, vol. 24, issue 4, Nov. 2004, pp. 281-290.
[18] M. Samadpour, J. E. Ongerth, J. Liston, N. Tran, D. Nguyen, T. S. Whittam, R. A. Wilson, and P.I. Tarr Occurrence of Shiga-like toxinproducing Escherichia coli in retail fresh seafood, beef, lamb, porkand poultry from grocery stores in Seattle, Washington. Applied and Environmental. Microbiology, vol. 60, No. 3, March. 1994, pp.1038- 1040.
[19] C. A. Philips. The epidemiology, detection and control of Escherichia coli O157. Journal of the Science of Food and Agriculture, vol. 79, issue 11, Aug. 1999, pp.1367-1381.
[20] J. S. Meng, T. Zhao, and M.P. Doyle Virulence genes of Shiga toxin producing Escherichia coli isolated from food, animals and humans. International Journal of Food Microbiology, vol. 45, issue 3 22, Dec. 1998, pp. 229-235.
[21] J. L. Holland, L. Louie, A. E. Simor, and M. Louie. PCR detection of Escherichia coli O157:H7 directly from stools: evaluatiuon of commercial extraction methods for purifying fecal DNA. Journal of Clinical Microbiology, vol. 38, issue 11, Nov. 2000, pp. 4108-4113.
[22] E. J. Kee, and R.O. Elder. (2002) Isolation of Shiga-toxigenic Escherichia coli O157 from hide surface and the oral cavity of finished beef feedlot cattle. Journal of the American Veterinary Medical Association, vol. 220, No. 6, March. 2002, pp.756-763.
[23] A. E. Heuvelink, F. E. Van Den Biggelaar, E. De Boer, R. G. Herbes, W. J. Melche, J. H. Huis, and L.A. Monnens L.A. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 strains from Dutch cattle and sheep. Journal of Clinical Microbiology, Vol.36, issue 4, Apr. 1998, pp. 878-882.
[24] G. A. Barkocy-Gallagher, T. M. Arthur, G.R. Siragusa, J. E. Keen, R. O. Elder, W.W. Laegreid, and M. Koohmaraie Genotypic analyses of Escherichia coli O157:H7 and O157nonmotil isolates recovered from beef cattle and carcasses at processing plants in the Midwestern of the United States. Applied and Environmental Microbiology, vol. 67, No. 9, Sept. 2001, pp. 3810-3818.
[25] P. A. Chapman, A. T. Cerdan Malo, M. Ellin, R. Ashton, and M.A. Harkin M.A. Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire,UK. International Journal of Food Microbiology, vol. 64, issue1/2 28, Febr. 2001, pp. 139-150.
[26] F. Rogerie, A. Marecat, S. Gambade, F. Dupond, P. Beaubois and M. Lange Characterization of Shiga toxin producing E. Coli and O157 serotype E. Coli isolated in France from healthy domestic cattle. International Journal of Food Microbiology, vol.63, issue 3, 15, Febr. 2001, pp. 217-223.
[27] E. M. Nielsen, and F. Scheutz. Characterisation of Escherichia coli O157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes. Veterinary Microbiology, vol. 88, issue 3, Sept. 2002, pp. 259-273.
[28] F. Omisakin, M. Macrae, I. D. Ogden, and N. J. Strachan Concentration and prevalence of Escherichia coli O157 in cattle feces at slaughter. Applied and Environmental. Microbiology, vol.69, No. 5, May. 2003, pp. 2444-2447.
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