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Determination of Cyclic Citrullinated Peptide Antibodies on Quartz Crystal Microbalance Based Nanosensors
In this study, we have focused our attention on combining of molecular imprinting into nanofilms and QCM nanosensor approaches and producing QCM nanosensor for anti- CCP, chosen as model protein, using anti-CCP imprinted nanofilms. The nonimprinted nanosensor was also prepared to evaluate the selectivity of the imprinted nanosensor. Anti-CCP imprinted QCM nanosensor was tested for real time detection of anti-CCP from aqueous solution. The kinetic and affinity studies were determined by using anti-CCP solutions with different concentrations. The responses related with mass shifts (%m) and frequency shifts (%f) were used to evaluate adsorption properties. To show the selectivity of the anti-CCP imprinted QCM nanosensor, competitive adsorption of anti-CCP and IgM was investigated. The results indicate that anti- CCP imprinted QCM nanosensor has higher adsorption capabilities for anti-CCP than for IgM, due to selective cavities in the polymer structure.
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[1] E. Yılmaz, L. Uzun, A.Y. Rad, U. Kalyoncu, S. Ünal, A. Denizli, “Specific adsorption of the autoantibodies from rheumatoid arthritis patient plasma using histidine-containing affinity beads,” J. Biomat. Sci- Polym E, vol. 19, pp. 875-892, 2008.
[2] M.A. Van Boekel, E.R. Vossenaar, F.H. van den Hoogen, W.J. van Venroo, “Autoantibody systems in rheumatoid arthritis: specificity, sensitivity and diagnostic value,” Arthritis Res, vol. 4, pp. 87-93, 2002.
[3] F. J. Diaz, A. Rojas-Villarraga, J. C. Salazar, A. Iglesias-Gamarra, R. D. Mantilla, J.M. Anaya, “Anti-CCP antibodies are associated with early age at onset in patients with rheumatoid arthritis,” Joint Bone Spine, vol. 78, pp. 175-178, 2011.
[4] J.L. Caro-Oleas, A. Fernández-Suárez, S. Reneses Cesteros, C. Porrino, A. Núñez-Roldán, I. Wichmann Schlipf, “Evaluation of third generation anti-CCP antibodies in the diagnosis of rheumatoid arthritis from undifferentiated polyarthritis after 4 years of follow-up,” Clin Exp Rheumatol, vol.26, pp. 461-463, 2008.
[5] N. Bereli, Y. Saylan, L. Uzun, R. Say, A. Denizli, “L-Histidine imprinted supermacroporous cryogels for protein recognition,” Sep Purif Technol, vol. 82, pp. 28-35, 2011.
[6] Y. Saylan, R. Üzek, L. Uzun, A. Denizli, “Surface imprinting approach for preparing specific adsorbent for IgG separation,” J. Biomat. Sci- Polym E, vol. 25, pp. 881-894, 2014.
[7] Y. Lv, T. Tan, F. Svec, “Molecular imprinting of proteins in polymers attached to the surface of nanomaterials for selective recognition of biomacromolecules,” Biotechnol. Adv, vol. 31, pp. 1172-1186, 2013.
[8] G. Sener, E. Özgur, E. Yılmaz, L. Uzun, R. Say, A. Denizli, “Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles,” Biosens Bioelectron, vol. 26, pp. 815-821, 2010.
[9] E. Yilmaz, D. Majidi, E. Ozgur, A. Denizli, “Whole cell imprinting based Escherichia coli sensors: A study for SPR and QCM,” Sensors Actuator, vol. 209, pp. 714-721, 2015.
[10] A. H. Wu, M. J. Syu, “Synthesis of bilirubin imprinted polymer thin film for the continuous detection of bilirubin in an MIP/QCM/FIA system,” Biosens Bioelectron, vol. 21, pp. 2345-2353, 2006.
[11] S. Emir Diltemiz, D. Hür, A. Ersöz, A. Denizli, R. Say, “Designing of MIP based QCM sensor having thymine recognition sites based on biomimicking DNA approach,” Biosensor Bioelectron, vol. 25 pp. 599- 603, 2009.
[12] K. Feng, J. Li, J. H.Jiang, G. L. Shen, R. Q. Yu, “QCM detection of DNA targets with single-base mutation based on DNA ligase reaction and biocatalyzed deposition amplification,” Biosens Bioelectron, vol. 22 pp. 1651-1657, 2007.
[13] A. Ersöz, S. Emir Diltemiz , A. Atılır Özcan, A. Denizli, R. Say, “8- OHdG sensing with MIP based solid phase extraction and QCM technique,” Sensors Actuator, vol. 137, pp. 7-11, 2009.
[14] I. Mannelli, M. Minunni, S. Tombelli, M. Mascini, “Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection,” Biosensor Bioelectron, vol. 18 pp. 129- 140, 2003.
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