Determination of Effective Variables on Arachidonic Acid Production by Mortierella alpina CBS 754.68in Solid-State Fermentation using Plackett-Burman Screening Design
In the present study, the oleaginous fungus
Mortierella alpina CBS 754.68 was screened for arachidonic
acidproduction using inexpensive agricultural by-products as
substrate. Four oilcakes were analysed to choose the best substrate
among them. Sunflower oilcake was the most effective substrate for
ARA production followed by soybean, colza and olive oilcakes. In
the next step, seven variables including substrate particle size,
moisture content, time, temperature, yeast extract supply, glucose
supply and glutamate supply were surveyed and effective variables
for ARA production were determined using a Plackett-Burman
screening design. Analysis results showed that time (12 days),
substrate particle size (1-1.4 mm) and temperature (20ºC) were the
most effective variables for the highest level of ARA production
 H. D. Jang, S. S. Yang. Polyunsaturated fatty acids production with a
solid-state column reactor. Bioresource Technol. 2008, 99: 6181-6189.
 M. H. Cheng, T. H. Walker, G. J. Hulbert and D. R. Raman. Fungal
production of eicosapentaenoic acid and arachidonic acids from
industrial waste streams and crude soybean oil. Bioresource Technol.
1999, 67: 101-110.
 S. Papanilolaou, M. Komaitis and G. Aggelis. Single cell oil (SCO)
production by Mortierella isabellina grown on high-sugar content media.
Bioresource Technol. 2004, 95: 287-291
 T. Aki, Y. Nagahata, K. Ishihara, Y. Tanaka, T. Morinaha, K.
Higashiyama, K. Akimoto, S. Fujikawa, S. Kawamoto, S. Shigeta, K.
ono and O. Suzuki. Production of arachidonic acid by filamentous
fangus, Mortierella alliacea strain YN-15. J Amer Oil Chem Soc. 2001,
 H. D. Jang, Y. Y. Lin and S. S. Yang. Polyunsaturated fatty acid
production with Mortierella alpina by solid substrate fermentation. Bot
Bull Acad Sin. 2000, 41: 41-48.
 M. Certik, L. Slavikova and J. Sajbidor. Enhancement of nutritional
value of cereals with ╬│-linolenic acid by fungal solid state fermentation.
Food Technol Biotechnol. 2006, 44(1): 75-82
 E. Conti, M. Stredansky, S. Stredanska and F. Zanetti. ╬│-Linolenic acid
production by solid-state fermentation of Mucorales strains on cereals.
Bioresourse Technol. 2001, 76: 283-286.
 H. Gema, A. Cavadia, D. Dimou, V. Tsagou, M. Komaitis and G.
Aggelis. Production of ╬│-linolenic acid by Cunninghamella echinulata
cultivated on glucose and orange peel. Appl Microbiol Biotechnol. 2002,
 P. Jangbua, K. Laoteng, P. Kitsubun, M. Nopharatana and A. Tongata.
Gamma-linolenic acid production of Mucor rouxii by solid-state
fermentation using agricultural by-products. Lett Appl Microbiol. 2009,
 M. Veen, C. lang. Production of lipid compounds in the yeast
Saccharomyces servisiae. Appl Microbiol Biotechnol. 2004, 63: 635-646
 M. Zhu, L.-J. Yu and Y.-X. Wu. An inexpensive medium for production
of arachidonic acid by Mortierella alpina. J Ind Microbiol Biotechnol.
2003, 30: 75-79.
 P. B. L. Fregolente, L. V. Fregolente, M. R. W. Maciel and P. O.
Carvalho. Screening of microbial lipases and evalutaion of their
potential to produce glycerides with high gamma linolenic acid
concentration. Braz J Microbiol. 2009, 40(4): 747-756
 M. Stradansky, E. Conti and A. Salaris. Production of polyunsaturated
fatty acids by Pythium ultimum in solid-stste cultivation. Enzyme
Microb Technol. 2000, 26: 304-307
 L. C. Metcalf, A. A. Schmitz, and J. R. Pelka. Rapid preparation of
methyl esters from lipid for gas chromatography analysis. Anal Chem.
1996, 38: 514-518.
 S. Stredanska, D. Slugen, M. Stredansky and J. Grego. Arachidonic acid
production by Mortierella alpina grown on solid substrates. World J
Microbiol Biotechnol. 1993, 9: 511-513.