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Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 29122

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In Vitro and Experimental Screening of Mangrove Herbal Extract against Vibrio Alginolyticus in Marine Ornamental Fish
Present study summarizes the control of Vibrio alginolyticus infection in hatchery reared Clownfish, Amphiprion sebae with the extract of the mangrove plant, Avicennia marina. Fishes with visible symptoms of hemorrhagic spots were chosen and the genomic DNA of the causative bacterium was isolated and sequenced based on 16S rDNA gene. The in vitro assay revealed that a fraction of A. marina leaf extract elucidated with ethyl acetate: methanol (6:4) showed a high activity (28 mm) at 125 μg/ml concentrations. About 4 % of the fraction fed along with live V. alginolyticus was significantly decreased the cumulative mortality (P<0.05) in the experimental groups than the control group. The responsible fraction was investigated by gas chromatography - mass spectroscopy and found the presence of active compounds. This is the first research in India to control vibriosis infection in marine ornamental fish with mangrove leaf extract.
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[1] T. T. Ajithkumar, and T. Balasubramanian, Broodstock development, spawning and larval rearing of the false clown fish, Amphiprion ocellaris in captivity using estuarine water. Curr. sci. vol. 97, no.10, 2009, 1483-1486.
[2] V. Inglis, R. J. Roberts, and N. R. Bromage, Bacterial Diseases of Fish. New York, NY, Halsted Press. 1993.
[3] Y. Danayadol, S. Direkbusarakom, and K. Supamattaya, Viral nervous necrosis in brown spotted grouper, E. malabaricus, cultured in Thailand, in: Shariff, M., Arthur, J.R., Subasinghe, R.P. (Eds.), Diseases in Asian aquaculture. Fish health section. Asian Fisheries Society, Manila, 1995, pp. 227-233.
[4] G.T. Rijkers, A.G. Teunissen, R. Van Oosteron, and W.B. Van Muiswinkel, The immune system of cyprinid fish: The immunosuppressive effects of the antibiotic oxytetracycline in carp (Cyprinus carpio L.). Aquaculture. 19, 1980, 177- 89.
[5] B. Robertsen, Modulation of the non-specific defence of fish by structurally conserved microbial polymers. Fish & Shellfish. immunl. 9, 1999, 269 - 290.
[6] M. T. Fauvel, J. T. Gleye, and I. Fouraste, Phenylpropanoid glycosides from Avicennia marina. Planta. Med. 59, 1993, 389.
[7] S. Kumaran, B. Deivasigamani, K. M. Alagappan, M. Sakthivel, and S. Guru Prasad, Isolation and characterization of Pseudomonas sp. KUMS3 from Asian sea bass (Lates calcarifer) with fin rot, World J. Microbiol. Biotechnol. 26, 2010, 359-363
[8] A.W Bauer, W. M. M. Kirby, J. C. Sherris and M. Truck, Antibiotic susceptibility testing by a standardised single disk method. American J. Clinical Pathol. 45, 1966, 493-496.
[9] NCCLS, Performance standards for antimicrobial disk susceptibility test, third ed. Approved standards, National Committee for Clinical Laboratory Standards, Villa Nova, 1993.
[10] M. Boonyaratpalin, Nutritional requirements of grouper Epinephelus. The Proceedings of Grouper Culture. National Institute of Coastal Aquaculture.Thailand, 1993, pp. 50-55.
[11] K. K. Lee, S. R. Yu, T. I. Yand, P. C. Liu, and F. R. Chen, Isolation and characterization of Vibrio alginolyticus from diseased kuruma prawn, Penaeus japonicus. Lett. Appl. Microbiol. 22, 1996, 111-114.
[12] K. Kathiresan, A review of studies on Pichavaram mangrove, southeast India. Hydrobiol. 430, 2000, 185-205.
[13] W. M. Bandaranayake, Bioactives, bioactive compounds and chemical constituents of mangrove plants. Wetland Ecol. Manage. 1, 2002, 42- 452.
[14] Y. Zhang, Y. Lu, L. Mao, P. Proksch, W. Lin, Tagalsins I and J, two novel tetraterpenoids from the mangrove plant, Ceriops tagal. Org. Lett. 7, 2005, 3037-3040.
[15] C. Pakhathirathien, C. Karalai, C. Ponglimanot, S. Subhadhirasakul, and K. Chantrapromma, Dammarane triterpenes from the hypocotyls and fruits of Ceriops tagal. J. Nat. Prod. 68, 2005, 1787-1789.
[16] L. He, Y.S. Wang, and Q.J. Wang, In vitro antitumor activity of triterpenes from Ceriops tagal. Nat. Prod. Res. 21, 2007, 1228-1233.
[17] J. Y. Wu, Q. Xiao, J. Xu, M.Y. Li, J.Y, Pan, and M. Yang, Natural products from true mangrove flora: source, chemistry and bioactivities. Nat. Prod. Rep. 25, 2008, 955-981.
[18] M. Chandrasekaran, V. Venkatesalu, S. Sivasankari, K. Kannathasan, A. K. Sajit, Khan, K. Prabhakar, S. Rajendran, and Y. Lakshmi Sarayu, Antibacterial activity of certain mangroves against methicillin resistant Staphylococcus aureus. Seaweed Res. Utiln. 28, 2006, 165-170.
[19] T. Itami, M. Asano, K. Tokushige, K. Kubono, A. Nakagawa, N. Takeno, H. Nishimura, M. Maeda, M. Kondo, and Y. Takahashi, Enhancement of disease resistance of kuruma shrimp, Penaeus japonicus after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture. 164, 1998, 277-288.
[20] T. Citarasu, R. R. Sekar, M. M. Babu, and M. P. Marian, Developing Artemia enriched herbal diet for producing quality larvae in Penaeus monodon. Asian Fish. Sci. 15, 2002, 21-32.
[21] M. M. Babu, and M. P. Marian, Developing bioencapsulated herbal products for maturation and quality larval production in Penaeus monodon (L.), in: Hendry, C., Van Stappen, G., Wille, M., Sorgeloos, P. (Eds.), 3rd fish and Shell fish Larviculture symposium. Spec. Publ. Eur. Aquacul Soc. 30, 2001, pp. 40-43.
[22] V. Jayaprakas, and J. Eupharsia, Growth performance of Labeo rohita (Ham.) Livol (IHF- 000), an herbal product. Proc. Ind. Natl. Sci. Acad. 63(2), 1996, 1-10.
[23] U. Kokpol, W. Chavasiri, V. Chittawong, and D.H. Miles, Taraxeryl cisp- hydroxycinnamate, a novel taraxeryl from Rhizophora mucronata. J. Nat. Prod. 53, 1990, 953-955.
[24] S. D. Killops, and N.L. Frewin, Triterpenoid diagenesis and cuticular preservation. Org. Geochem. 21, 1994, 1193-1209.
[25] B.P. Koch, J. Rullkotter, and R.J. Lara, Evaluation of triterpenols and sterols as organic matter biomarkers in a mangrove ecosystem in northern Brazil. Wetl. Ecol. Manag. 11, 2003, 257-263.
[26] G. J. M. Versteegh, E. Scheful, L. Dupont, F. Marret, J. S. S. Damste, and J. H. R. Jansen, Taraxerol and Rhizophora pollen as proxies for tracking past mangrove ecosystems. Geochim. Cosmochim. Acta. 68, 2004, 411-422.
[27] S. Laphookhieo, C Karalai, and C Ponglimanont, New sesquiterpenoid and triterpenoids from the fruits of Rhizophora mucronata. Chem. Pharm. Bull. 52, 2004, 883-885.

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