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Modified Poly(pyrrole) Film Based Biosensors for Phenol Detection
In order to detect and quantify the phenolic contents of a wastewater with biosensors, two working electrodes based on modified Poly(Pyrrole) films were fabricated. Enzyme horseradish peroxidase was used as biomolecule of the prepared electrodes. Various phenolics were tested at the biosensor. Phenol detection was realized by electrochemical reduction of quinones produced by enzymatic activity. Analytical parameters were calculated and the results were compared with each other.
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[1] T. Ruzgas, E. Csöregi, J. Emneus, L. Gorton, G. Makro-Varga, “Peroxidase-modified electrodes: Fundamentals and application,” Anal. Chim. Acta, vol. 330, pp. 123-138, September 1996.
[2] J. Yu, S. Liu, H. Ju, “Mediator-free phenol sensor based on titania solgel encapsulation matrix for immobilization of tyrosinase by a vapor deposition method,” Biosens. Bioelectron., vol. 19, pp. 509-514, December 2003.
[3] C. Capannesi, I. Ilaria Palchetti, M. Macsini, A. Parenti, “Electrochemical sensor and biosensor for polyphenols detection in olive oils,” Food Chemistry, vol. 71, pp. 553-562, December 2000.
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[5] S. Rodriguez-Mozaz, M. J. Lopez de Alda, M. P. Marco, D. Barcelo, “Biosensors for environmental monitoring,” Talanta, vol. 65, pp. 291- 297, January 2005.
[6] Y. L. Zhaou, R. H. Tian, J. F. Zhi, “Amperometric biosensor based on tyrosinase immoblized on a boron-doped diamond electrode,” Biosens. Bioelectron., vol. 22, pp. 822-828, January 2007.
[7] S. C. Chang, K. Rawson, C. J. McNeil, “Disposable tyrosinaseperoxidase bi-enzyme sensor for amperometric detection of phenols,” Biosens. Bioelectron., vol. 17, pp. 1015-1023, December 2002.
[8] A. Lindgren, J. Emn´eus, T. Ruzgas, L. Gorton, G. Marko- Varga, “Amperometric detection of phenols using peroxidase-modified graphite electrodes,” Anal. Chim. Acta, vol. 347, pp. 51-62, July 1997.
[9] Y. C. Tsai, C. Cheng-Chiu, “Amperometric biosensors based on multiwalled carbon nanotube-Nafion-tyrosinase nanobiocomposites for the determination of phenolic compounds,” Sens. Actuators B: Chem., vol. 125, pp. 10-16, July 2007.
[10] Q. Fan, D. Shan, H. Xue, Y. He, S. Cosnier, “Amperometric phenol biosensor based on laponite-clay chitosan nanocomposite matrix,” Biosens. Bioelectron., vol. 22, pp. 816-821, January 2007.
[11] Rajesh, K. Kaneto, “A new tyrosinase biosensor based on covalent immobilization of enzyme on N-(3-aminopropyl) pyrrole polymer film,” Curr. Appl. Phys., vol. 5, pp. 178-183, February 2005.
[12] S. Korkut Ozoner, M. Yalvac Can, B. Keskinler, E. Erhan, “A novel Poly(gluteraldehyde-co-pyrrole)/horseradish peroxidase composite film electrode,” Anal. Lett., vol. 42, pp. 3058-3072, November 2009.
[13] S. Korkut Ozoner, “Poly(glycidyl methacrylate-co-3-thienylmethyl methacrylate based working electrodes for hydrogenperoxide biosensing,” J Chem. Technol. Biotechnol., vol. 87, pp. 146-152, January 2012.
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