Open Science Research Excellence
%0 Journal Article
%A Ahmad Manbohi and  Seyyed Hamid Ahmadi 
%D 2017 
%J  International Journal of Mechanical, Aerospace, Industrial, Mechatronic and Manufacturing Engineering
%B World Academy of Science, Engineering and Technology
%I International Science Index 131, 2017
%T Microfluidic Paper-Based Electrochemical Biosensor
%V 131
%X A low-cost paper-based microfluidic device (PAD) for the multiplex electrochemical determination of glucose, uric acid, and dopamine in biological fluids was developed. Using wax printing, PAD containing a central zone, six channels, and six detection zones was fabricated, and the electrodes were printed on detection zones using pre-made electrodes template. For each analyte, two detection zones were used. The carbon working electrode was coated with chitosan-BSA (and enzymes for glucose and uric acid). To detect glucose and uric acid, enzymatic reactions were employed. These reactions involve enzyme-catalyzed redox reactions of the analytes and produce free electrons for electrochemical measurement. Calibration curves were linear (R² > 0.980) in the range of 0-80 mM for glucose, 0.09–0.9 mM for dopamine, and 0–50 mM for uric acid, respectively. Blood samples were successfully analyzed by the proposed method.

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