Open Science Research Excellence
%0 Journal Article
%A Patrick Nwabueze Okechukwu and  Ima Nirwana Soelaiman and  Gabriele Anisah Ruth Froemming and  Mohd Yusri Idorus and  Norazlina Mohamed
%D 2012 
%J  International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering
%B World Academy of Science, Engineering and Technology
%I International Science Index 72, 2012
%T Bone Proteome Study in Ovariectomised Rats Supplemented with Palm Vitamin E
%V 72
%X Supplementation of palm vitamin E has been reported
to prevent loss of bone density in ovariectomised female rats. The
mechanism by which palm vitamin E exerts these effects is still
unknown. We hypothesized that palm vitamin E may act by
preventing the protein expression changes. Two dimensional poly
acyrilamide gel electrophoresis (2-D PAGE) and PD Quest software
genomic solutions Investigator (proteomics) was used to analyze the
differential protein expression profile in femoral and humeri bones
harvested from three groups of rats; sham-operated rats (SO),
ovariectomised rats (Ovx) and ovariectomised rats supplemented for
2 months with palm vitamin E. The results showed that there were
over 300 valued spot on each of the groups PVE and OVX as
compared to about 200 in SO. Comparison between the differential
protein expression between OVX and PVE groups showed that ten
spots were down –regulated in OVX but up-regulated in PVE. The
ten differential spots were separately named P1-P10. The
identification and understanding of the pathway of the differential
protein expression among the groups is ongoing and may account for
the molecular mechanism through which palm vitamin E exert its
anti-osteoporotic effect.
%P 728 - 732