Open Science Research Excellence
%0 Journal Article
%A L. Fonseca-Géigel and  M. Alvarez and  G. García and  R. Cox and  L. Morier and  L. Fonte and  M. G. Guzmán
%D 2011 
%J  International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering
%B World Academy of Science, Engineering and Technology
%I International Science Index 52, 2011
%T Plaque Formation of Toxoplasma gondii in Vero Cells using Carboxymethylcellulose
%V 52
%X Toxoplasma gondii is an intracellular parasite capable
of infecting all nucleated cells in a diverse array of species.
Toxoplasma plaque assay have been described using Bacto Agar.
Because of its experimental advantages carboxymethyl cellulose
overlay, medium viscosity was choosing and the aim of this work
was to develop alternative method for formation of T. gondii plaques.
Tachyzoites were inoculated onto monolayers of Vero cells and
cultured at 37° C under 5 % CO2. The cultures were followed up by
microscopy inspection. Small plaques were visible by naphtol blue
stain 4 days after infection. Larger plaques could be observed by day
10 of culture. The carboxymethyl cellulose is a cheap reagent and the
methodology is easier, faster than assays under agar overlay. This is
the first description of the carboxymethyl cellulose overlay use for
obtaining the formation of T. gondii plaques and may be useful in
consequent obtaining tachyzoites for detailed studies.
%P 194 - 196