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@article{(International Science Index):http://waset.org/publications/4123,
  title    = {Changes of in vitro Cytokine Production induced by δ-Lactams},
  author    = {Y. Baba hamed and  A. Medjdoub and  H. Merzouk and  M. Narce},
  country   = {},
  institution={},
  abstract  = {The aim of this work was to study the in vitro effects
of δ-lactam 1 and its 4-chlorophenyl derivative 2, on the proliferative
responses of human lymphocytes and Th1 and Th2 cytokine
secretion. The possible protective role of vitamin E on intracellular
stress oxidative induced by these compounds was also investigated.
Peripheral blood lymphocytes were isolated using differential
centrifugation on a density gradient of Histopaque. They were
cultured with mitogen concanavalin A, vitamin E (10 μM) and with
different concentrations of the compounds 1 and 2 (0.1 to 10 μM).
Proliferation (MTT assay), IL-2, INFγ and IL-4 (Elisa kits),
intracellular superoxide anion were determined. 1 and 2 were
immunostimulant and increased cytokine secretion with a shift away
from Th1 response to Th2. These properties were however
accompanied by an increase in intracellular oxidative stress. The
presence of vitamin E exhibited protective effects by reducing δ-
lactam-induced superoxide anion generation in lymphocytes.},
    journal   = {International Journal of Chemical, Molecular, Nuclear, Materials and Metallurgical Engineering},  volume    = {6},
  number    = {8},
  year      = {2012},
  pages     = {693 - 697},
  ee        = {http://waset.org/publications/4123},
  url       = {http://waset.org/Publications?p=68},
  bibsource = {http://waset.org/Publications},
  issn      = {PISSN:2010-376X, EISSN:2010-3778},
  publisher = {World Academy of Science, Engineering and Technology},
  index     = {International Science Index 68, 2012},
}