Durian is the flagship fruit of Mindanao and there is
an abundance of several cultivars with many confusing identities/
The project was conducted to develop procedure for reliable and
rapid detection and sorting of durian planting materials. Moreover, it
is also aimed to establish specific genetic or DNA markers for routine
testing and authentication of durian cultivars in question.
The project developed molecular procedures for routine testing.
SSR primers were also screened and identified for their utility in
discriminating durian cultivars collected.
Results of the study showed the following accomplishments:
1. Twenty (29) SSR primers were selected and identified based on
their ability to discriminate durian cultivars,
2. Optimized and established standard procedure for identification
and authentication of Durian cultivars
3. Genetic profile of durian is now available at Biotech Unit
Our results demonstrate the relevance of using molecular
techniques in evaluating and identifying durian clones. The most
polymorphic primers tested in this study could be useful tools for
detecting variation even at the early stage of the plant especially for
commercial purposes. The process developed combines the efficiency
of the microsatellites development process with the optimization of
non-radioactive detection process resulting in a user-friendly protocol
that can be performed in two (2) weeks and easily incorporated into
laboratories about to start microsatellite development projects. This
can be of great importance to extend microsatellite analyses to other
crop species where minimal genetic information is currently
available. With this, the University can now be a service laboratory
for routine testing and authentication of durian clones.
 Nafsi N.I. 2007. Diversity Analysis of Durian (Durio zibethinus Murr.)
Varieties using Microsatellite Markers. (Thesis). School of Bioscience
and Biotechnology, Bandung, Institute of Technology, Bandung,
 Baswarsiati, Yuniarti, Suhardi, Harwanto, Rahmawari D., Seogiyarto M.
2007. Characterization of Some Properties of Durian Germplasm in
Kediri District. Assessment Institute for Agricultural Technology
(BPTP), East Java Province, Surabaya, Indonesia.
 Tacca, J.A., Abad, R.G. and Bastian, S.T. Jr. 2005. Molecular
Characterization and Relationships of 14 Durian Cultivars (Durio
zibethinus Murr.) using RAPD markers. Scientific Conference of the
Federation of Crop science Societies of the Philippines, Lapasan,
Cagayan de Oro City, Philippines. 30:20.
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