Excellence in Research and Innovation for Humanity

International Science Index

Select areas to restrict search in scientific publication database:
Ethanol and Biomass Production from Spent Sulfite Liquor by Filamentous Fungi
Since filamentous fungi are capable of assimilating several types of sugars (hexoses and pentoses), they are potential candidates for bioconversion of spent sulfite liquor (SSL). Three filamentous fungi such as Aspergillus oryzae, Mucor indicus, and Rhizopus oryzae were investigated in this work. The SSL was diluted in order to obtain concentrations of 50, 60, 70, 80, and 90% and supplemented with two types of nutrients. The results from cultivations in shake flask showed that A. oryzae and M. indicus were not able to grow in pure SSL and SSL90% while R. oryzae could grow only in SSL50% and SSL60%. Cultivation with A. oryzae resulted in the highest yield of produced fungal biomass, while R. oryzae cultivation resulted in the lowest fungal biomass yield. Although, the mediums containing yeast extract, (NH4)2SO4, KH2PO4, CaCl2∙2H2O, and MgSO4∙7H2O as nutrients supplementations produced higher fungal biomass compared to the mediums containing NH4H2PO4 and ammonia, but there was no significant difference between two types of nutrients in terms of sugars and acetic acid consumption rate. The sugars consumption in M. indicus cultivation was faster than A. oryzae and R. oryzae cultivation. Acetic acid present in SSL was completely consumed during cultivation of all fungi. M. indicus was the best and fastest ethanol producer from SSL among the fungi examined, when yeast extract and salts were used as nutrients supplementations. Furthermore, no further improvement in ethanol concentration and rate of sugars consumption was obtained in medium supplemented with NH4H2PO4 and ammonia compared to medium containing yeast extract, (NH4)2SO4, KH2PO4, CaCl2∙2H2O, and MgSO4∙7H2O. On the other hand, the higher dilution of SSL resulted in a better fermentability, and better consumption of sugars and acetic acid.
Digital Article Identifier (DAI):


[1] S. R. Pereira, D. J. Portugal-Nunes, D. V. Evtuguin, L. S. Serafim, and A. M. R. B. Xavier, “Advances in ethanol production from hardwood spent sulphite liquors,” Process Biochemistry, vol. 48, no. 2, pp. 272–282, Feb. 2013.
[2] V. Novy, S. Krahulec, K. Longus, M. Klimacek, and B. Nidetzky, “Co-fermentation of hexose and pentose sugars in a spent sulfite liquor matrix with genetically modified Saccharomyces cerevisiae,” Bioresource Technology, vol. 130, pp. 439–448, Feb. 2013.
[3] A. M. R. B. Xavier, M. F. Correia, S. R. Pereira, and D. V. Evtuguin, “Second-generation bioethanol from eucalypt sulphite spent liquor,” Bioresource Technology, vol. 101, no. 8, pp. 2755–2761, Apr. 2010.
[4] D. L. A. Fernandes, C. M. Silva, A. M. R. B. Xavier, and D. V. Evtuguin, “Fractionation of sulphite spent liquor for biochemical processing using ion exchange resins,” Journal of Biotechnology, vol. 162, no. 4, pp. 415– 421, Dec. 2012.
[5] M. Weissgram, Ch. Herwig, and H. K. Weber, “Biotechnological Generation of Value Added Products from Spent Pulping Liquors: Assessing the Potential of Extremophiles,” J. Bioprocessing and Biotechniques, vol. 5, no. 7, pp. 1-14, July 2015.
[6] T. Llano, C. Rueda, N. Quijorna, A. Blanco, and A. Coz, “Study of the delignification of hardwood chips in a pulping process for sugar production,” Journal of Biotechnology, vol. 162, no. 4, pp. 422– 429, Dec. 2012.
[7] S. R. Pereira, Š. Ivanuša, D. V. Evtuguin, L. S. Serafim, and A. M. R. B. Xavier, “Biological treatment of eucalypt spent sulphite liquors: A way to boost the production of second generation bioethanol,” Bioresource Technology, vol. 103, no. 1, pp. 131–135, Jan. 2012.
[8] Zh. Guo, and L. Olsson, “Characterization and fermentation of side streams from sulfite pulping,” Process Biochemistry, vol. 49, no. 8, pp. 1231–1237, Aug. 2014.
[9] S. S. Helle, T. Lin, and Sh. J. B. Duff, “Optimization of spent sulfite liquor fermentation,” Enzyme and Microbial Technology, vol. 42, no. 3, pp. 259–264, Feb. 2008.
[10] E. Johansson, T. Brandberg, and C. Larsson, “Influence of cultivation procedure for Saccharomyces cerevisiae used as pitching agent in industrial spent sulphite liquor fermentations,” Journal of Industrial Microbiology and Biotechnology, vol. 38, no. 11, pp. 1787–1792, Nov. 2011.
[11] R. Millati, L. Edebo, and M. J. Taherzadeh, “Performance of Rhizopus, Rhizomucor, and Mucor in ethanol production from glucose, xylose, and wood hydrolyzates,” Enzyme and Microbial Technology, vol. 36, no. 2-3, pp. 294–300, Feb. 2005.
[12] M. Holmgren, and A. Sellstedt, “Identification of white-rot and soft-rot fungi increasing ethanol production from spent sulfite liquor in co-culture with Saccharomyces cerevisiae,” Journal of Applied Microbiology, vol. 105, no. 1, pp. 134–140, Jul. 2008.
[13] J. A. Ferreira, P. R. Lennartsson, C. Niklasson, M. Lundin, L. Edebo, and M. J. Taherzadeh, “Spent Sulphite Liquor for Cultivation of an Edible Rhizopus SP,” BioResources, vol. 7, no. 1, pp. 173- 188, Nov. 2012.
[14] H. A. El-Enshasy, “Bioprocessing for Value-Added Products from Renewable Resources: New Technologies and Applications, Chapter 9. Filamentous Fungal Cultures – Process Characteristics, Products, and Applications,” ISBN: 978-0-444-52114-9, 2007, p. 225.
[15] K. Karimi, G. Emtiazi, and M. J. Taherzadeh, “Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae,” Enzyme and Microbial Technology, vol. 40, no. 1, pp. 138–144, Dec. 2006.
[16] J. A. Ferreira, P. R. Lennartsson, and M. J. Taherzadeh, “Production of Ethanol and Biomass from Thin Stillage Using Food-Grade Zygomycetes and Ascomycetes Filamentous Fungi,” Energies, vol. 7, no. 6, pp. 3872-3885, June 2014.
[17] V. Bátori, J. A. Ferreira, M. J. Taherzadeh, and P. R. Lennartsson, “Ethanol and Protein from Ethanol Plant By-Products Using Edible Fungi Neurospora intermedia and Aspergillus oryzae,” BioMed Research International, 2015, to be published.
[18] M. J. Taherzadeh, M. Fox, H. Hjorth, and L. Edebo, “Production of mycelium biomass and ethanol from paper pulp sulfite liquor by Rhizopus oryzae,” Bioresource Technology, vol. 88, no. 3, pp. 167–177, Jul. 2003.
[19] E. Casey, M. Sedlak, N. W. Y. Ho and N. S. Mosier, “Effect of acetic acid and pH on the co-fermentation of glucose and xylose to ethanol by a genetically engineered strain of Saccharomyces cerevisiae,” FEMS Yeast Research, vol. 10, no. 4, pp. 385–393, June 2010.
[20] W. Zheng-yun, D. Yu, T. Li, L. Yue-hong, Z. Yi-jie, and Zh. Wen-xue, “Investigating the effects of two lignocellulose degradation by-products (furfural and acetic acid) on ethanol fermentations by six ethanologenic yeast strains,” African Journal of Biotechnology, vol. 9, no. 50, pp. 8661-8666, Dec. 2010.
[21] D. Greetham, “Presence of Low Concentrations of Acetic Acid Improves Fermentations using Saccharomyces cerevisiae,” Journal of Bioprocessing and Biotechniques, vol. 5, no. 1, pp. 1-5, Dec. 2014.
Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007