Excellence in Research and Innovation for Humanity

International Science Index


Select areas to restrict search in scientific publication database:
10006265
In vitro Effects of Viscum album on the Functionality of Rabbit Spermatozoa
Abstract:
This study aimed to assess the in vitro effects of different concentrations of the Viscum album extract on the motility, viability, and reactive oxygen species (ROS) production by rabbit spermatozoa during different time periods (0, 2, and 8h). Spermatozoa motility was assessed by using the CASA (Computer aided sperm analysis) system. Cell viability was evaluated by using the metabolic activity MTT assay, and the luminol-based luminometry was applied to quantify the ROS formation. The CASA analysis revealed that low Viscum concentrations were able to prevent a rapid decline of spermatozoa motility, especially in the case of concentrations ranging between 1 and 5 µg/mL (P<0.05 with respect to time 8h). At the same time, concentrations ranging between 1 and 100 µg/mL of the extract led to a significant preservation of the cell viability (P<0.05 in case of 5, 50 and 100 µg/mL; P<0.01 with respect to 1 and 10 µg/mL, time 8h). 1 and 5 µg/mL of the extract exhibited antioxidant characteristics, translated into a significant reduction of the ROS production, particularly notable at time 8h (P<0.01). The results indicate that the Viscum extract is capable of delaying the damage inflicted to the spermatozoon by the in vitro environment.
Digital Article Identifier (DAI):

References:

[1] F.M. Lanzafame, S. La Vignera, E. Vicari, and A.E. Calogero, “Oxidative stress and medical antioxidant treatment in male infertility,” Reproductive Biomedicine Online, vol. 19, pp. 638-659, 2009.
[2] A. K. Bansal, and G. S. Bilaspuri, “Impacts of oxidative stress and antioxidants on semen functions,” Veterinary Medicine International, 2011, doi:10.4061/2011/686137.
[3] W. T. Holt, “Fundamental aspects of sperm cryobiology: the importance of species and individual differences,” Theriogenology, vol. 53, pp. 47-58, 2000.
[4] C. Alarcon de la Rastra, “Curcumin: a promising spice for therapeutics,” Molecular Nutrition & Food Research, vol. 52, pp. 985, 2008.
[5] M. Jurin, N. Zarkovic, M. Hrzenjak, and Z. Hic, Z., “Antitumorous and immunomodulatory effects of the Viscum album L. preparation Isorel,” Oncology, vol. 50, pp. 393-398, 1993.
[6] P.O. Osadebe, and I. C. Uzochukwu, “Chromatographic and anti-motility studies on extracts of Loranthus micranthus Linn,” Journal of Pharmaceutical and Allied Sciences, vol. 3, pp. 263-268, 2006.
[7] N. Jolanta, and O. Przemysław, “Phytochemical profile and therapeutic potential of Viscum album L.,” Natural Product Research, vol. 30, pp. 373-385, 2016.
[8] R. Grossarth-Maticek, and R. Ziegler, “Prospective controlled cohort studies on long-term therapy of ovarian cancer patients with mistletoe (Viscum album L.) extracts iscador,” Arzneimittelforschung, vol. 57, pp. 665-78, 2007.
[9] M. Kačániová, D. Ďurechová, N, Vuković, A. Kántor, J. Petrová, L. Hleba, and A. Vatľák, “Antimicrobial activity of Drosera rotundifolia L.,” Scientific Papers: Animal Science and Biotechnologies, vol. 47, pp. 366-369, 2014.
[10] E. Tvrdá, N. Lukáč, J. Lukáčová, T. Jambor, and P. Massányi, “Dose- and time-dependent in vitro effects of divalent and trivalent iron on the activity of bovine spermatozoa,” Biological Trace Element Research, vol. 167, pp. 36-47, 2015.
[11] S. I. Moskovstev, and C. L. Librach, “Methods of sperm vitality assessment,” in: Spermatogenesis, Methods and Protocols, 1st ed. vol. 927, D. T. Carrel, and K. I. Aston, Ed. New York: Springer Science + Business Media, pp. 13-19.
[12] A. H. Kashou, R. Sharma, and A. Agarwal, “Assessment of oxidative stress in sperm and semen,” Methods in Molecular Biology, vol. 927, 351-361, 2013.
[13] E. Tvrdá, E. Tušimová, A. Kováčik, D. Paál, H. Greifová, A. Abdramanov, and N. Lukáč, “Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress,” Animal Reproduction Science, 2016, in press.
[14] G. Kuttan, D. M. Vasudevan, and R. Kuttan, “Effect of a preparation from Viscum album on tumor development in vitro and in mice,” Journal of Ethnopharmacology, vol. 29, pp. 35-41, 1990
[15] U. Weissenstein, M. Kunz, K. Urech, and S. Baumgartner, “Interaction of standardized mistletoe (Viscum album) extracts with chemotherapeutic drugs regarding cytostatic and cytotoxic effects in vitro,” BMC Complementary and Alternative Medicine, vol. 14, pp. 6, 2014.
[16] E. Kovacs, “The in vitro effect of Viscum album (VA) extract on DNA repair of peripheral blood mononuclear cells (PBMC) in cancer patients,” Phytotherapy Research, vol. 16, pp. 143-147, 2002.
[17] M. O. Ojezele, E. O. Erhirhie, and O. A. Arojojoye, “Effects of Viscum album (mistletoe) from three host plants (cocoa, kola and coffee) on semen quality of wistar albino rats,” Chemistry International, vol. 3, pp. 109-113, 2017.
[18] L. Mazzi, M. Geminiani, G. Collodel, F. Iacoponi, S. Martini, C. Bonechi, C. Rossi, and E. Moretti, “Quercetin and rutin: effects of two flavonoids on induced oxidative stress in human ejaculated sperm,” Journal of Sienna Academy of Science, vol. 3, pp. 22-26, 2011.
[19] P.H. Purdy, S.A. Ericsson, R.E. Dodson, K.L. Sternes, and D.L. Garner, “Effects of the flavonoids, silibinin and catechin, on the motility of extended cooled caprine sperm,” Small Ruminant Research, vol. 55, pp. 239-243, 2004.
[20] N. H. Tung, Y. Shoyama, M. Wada, and H. Tanaka, “Improved in vitro fertilization ability of mouse sperm caused by the addition of Licorice extract to the preincubation medium,” The Open Reproductive Science Journal, vol. 6, pp. 1-7, 2014.
[21] O. E. Ofem, A. B. Antai, N. M. Essien, and V. O. Oka, “Enhancement of some sex hormones concentrations by consumption of leaves extract of Viscum album (mistletoe) in rats,” Asian Journal of Medical Sciences, vol. 5, pp. 87-90, 2014.
[22] V. Katalinic, M. Milos, T. Kulisic, and M. Jukic, “Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols,” Food Chemistry, vol. 94, pp. 550-557, 2006.
[23] Ch. Proestos, K. Lytoudi, O. K. Mavromelanidou, P. Zoumpoulakis, and V. J. Sinanoglou, “Antioxidant capacity of selected plant extracts and their essential oils,” Antioxidants, vol. 2, pp. 11-22, 2013.
[24] R. L. Stan, A. C. Hangan, L. Dican, B. Sevastre, D. Hanganu, C. Catoi O. Sarpataki, and C. M. Ionescu, “Comparative study concerning mistletoe viscotoxins antitumor activity,” Acta Biologica Hungarica, vol. 64, pp. 279-288, 2013.
[25] W. H. Kim, W. B. Park, B. Gao, and M. H. Jung, “Critical role of reactive oxygen species and mitochondrial membrane potential in Korean mistletoe lectin-induced apoptosis in human hepatocarcinoma cells,” Molecular Pharmacology, vol. 66, pp. 1383-1396, 2004.
[26] B. K. Kim, M. J. Choi, K. Y. Park, and E. J. Cho, “Protective effects of Korean mistletoe lectin on radical-induced oxidative stress,” Biological and Pharmaceutical Bulletin, vol. 33, pp. 1152-1158, 2010.
[27] A. Ateşşahin, G. Türk, S. Yilmaz, M. Sönmez, F. Sakin, and A. O. Ceribasi, “Modulatory effects of lycopene and ellagic acid on reproductive dysfunction induced by polychlorinated biphenyl (Aroclor 1254) in male rats,” Basic & Clinical Pharmacology & Toxicology, vol. 106, pp. 479-89, 2010.
[28] A. O. Çeribaşı, F. Sakin, G. Türk, M. Sönmez, and A. Ateşşahin, “Impact of ellagic acid on adriamycin-induced testicular histopathological lesions, apoptosis, lipid peroxidation and sperm damages,” Experimental and Toxicologic Pathology, vol. 64, pp. 717-724, 2012.
Vol:12 No:06 2018Vol:12 No:05 2018Vol:12 No:04 2018Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007