Excellence in Research and Innovation for Humanity

International Science Index


Select areas to restrict search in scientific publication database:
10007041
Synthesis of Highly Sensitive Molecular Imprinted Sensor for Selective Determination of Doxycycline in Honey Samples
Abstract:
Doxycycline (DXy) is a cycline antibiotic, most frequently prescribed to treat bacterial infections in veterinary medicine. However, its broad antimicrobial activity and low cost, lead to an intensive use, which can seriously affect human health. Therefore, its spread in the food products has to be monitored. The scope of this work was to synthetize a sensitive and very selective molecularly imprinted polymer (MIP) for DXy detection in honey samples. Firstly, the synthesis of this biosensor was performed by casting a layer of carboxylate polyvinyl chloride (PVC-COOH) on the working surface of a gold screen-printed electrode (Au-SPE) in order to bind covalently the analyte under mild conditions. Secondly, DXy as a template molecule was bounded to the activated carboxylic groups, and the formation of MIP was performed by a biocompatible polymer by the mean of polyacrylamide matrix. Then, DXy was detected by measurements of differential pulse voltammetry (DPV). A non-imprinted polymer (NIP) prepared in the same conditions and without the use of template molecule was also performed. We have noticed that the elaborated biosensor exhibits a high sensitivity and a linear behavior between the regenerated current and the logarithmic concentrations of DXy from 0.1 pg.mL−1 to 1000 pg.mL−1. This technic was successfully applied to determine DXy residues in honey samples with a limit of detection (LOD) of 0.1 pg.mL−1 and an excellent selectivity when compared to the results of oxytetracycline (OXy) as analogous interfering compound. The proposed method is cheap, sensitive, selective, simple, and is applied successfully to detect DXy in honey with the recoveries of 87% and 95%. Considering these advantages, this system provides a further perspective for food quality control in industrial fields.

References:

[1] T. Le, H. Yu, Y. Guo, B. Ngom, Y. Shen, and D. Bi, “Development of an indirect competitive ELISA for the detection of doxycycline residue in animal edible tissues,” Food Agric. Immunol., vol. 20, no. 2, pp. 111–124, 2009.
[2] A. Husbandry, “Veterinary Bureau of Ministry of Agriculture. 2003. Announcement of Ministry of Agriculture maximum residue levels of veterinary drug in foodstuffs of animal origin,” Chin. J. Vet. Drug, vol. 4, pp. 15–20.
[3] R. Mahmoudi, A. Ghojoghi, and P. Ghajarbeygi, “Honey Safety Hazards and Public Health,” J. Chem. Health Risks, vol. 6, no. 4, 2016.
[4] P. J. Ramesh, K. Basavaiah, K. Tharpa, K. B. Vinay, and H. D. Revanasiddappa, “Development and validation of RP-HPLC method for the determination of doxycycline hyclate in spiked human urine and pharmaceuticals,” J. Pre-Clin. Clin. Res., vol. 4, no. 2, 2010.
[5] N. Ruz, M. Zabala, M. G. Kramer, M. A. Campanero, M. C. Dios-Viéitez, and M. J. Blanco-Prı́eto, “Rapid and simple determination of doxycycline in serum by high-performance liquid chromatography: Application to particulate drug delivery systems,” J. Chromatogr. A, vol. 1031, no. 1, pp. 295–301, 2004.
[6] T. Charoenraks, S. Palaharn, K. Grudpan, W. Siangproh, and O. Chailapakul, “Flow injection analysis of doxycycline or chlortetracycline in pharmaceutical formulations with pulsed amperometric detection,” Talanta, vol. 64, no. 5, pp. 1247–1252, 2004.
[7] Z. M. Chen, G. H. Chen, Q. H. Hao, Z. Wang, and Y. X. Guo, “Determination of Doxycycline Residue in Pork by Derivative Synchronous Fluorimetry,” J. Hebei Univ. Nat. Sci. Ed., vol. 31, no. 1, pp. 57–62, 2011.
[8] X. Chen, W. Liang, C. Yang, W. Lin, and M. Bi, “Simultaneous quantitative detection of tetracyclines derivatives by raman spectroscopy,” in 2012 IEEE International Conference on Virtual Environments Human-Computer Interfaces and Measurement Systems (VECIMS) Proceedings, 2012, pp. 111–114.
[9] M. S. Attia, “Spectrofluorimetric assessment of Ramipril using optical sensor Samarium ion–doxycycline complex doped in sol–gel matrix,” J. Pharm. Biomed. Anal., vol. 51, no. 1, pp. 7–11, 2010.
[10] A. C. Kogawa, N. P. de Mello, and H. R. N. Salgado, “Quantification of Doxycycline in Raw Material by an Eco-Friendly Method of Infrared Spectroscopy,” Pharm. Anal. Acta, vol. 2016, 2016.
[11] J. Adrian, F. Fernández, F. Sánchez-Baeza, and M.-P. Marco, “Preparation of antibodies and development of an enzyme-linked immunosorbent assay (ELISA) for the determination of doxycycline antibiotic in milk samples,” J. Agric. Food Chem., vol. 60, no. 15, pp. 3837–3846, 2012.
[12] L. I. Andersson, “Molecular imprinting for drug bioanalysis: a review on the application of imprinted polymers to solid-phase extraction and binding assay,” J. Chromatogr. B. Biomed. Sci. App., vol. 739, no. 1, pp. 163–173, 2000.
[13] S. A. Piletsky and A. P. Turner, “Electrochemical sensors based on molecularly imprinted polymers,” Electroanalysis, vol. 14, no. 5, pp. 317–323, 2002.
[14] S. Li, J. Li, Q. Lin, and X. Wei, “A molecularly imprinted sensor based on an electrochemiluminescent membrane for ultratrace doxycycline determination,” Analyst, vol. 140, no. 13, pp. 4702–4707, 2015.
[15] A. H. Kamel, F. T. Moreira, F. Sales, and M. Goreti, “Biomimetic sensor potentiometric system for doxycycline antibiotic using a molecularly imprinted polymer as an artificial recognition element,” Sens. Lett., vol. 9, no. 5, pp. 1654–1660, 2011.
Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007