The Kinetic of Biodegradation Lignin in Water Hyacinth (Eichhornia Crassipes) by Phanerochaete Chrysosporium using Solid State Fermentation (SSF) Method for Bioethanol Production, Indonesia
Lignocellulosic materials are considered the most
abundant renewable resource available for the Bioethanol
Production. Water Hyacinth is one of potential raw material of the
world-s worst aquatic plant as a feedstock to produce Bioethanol.
The purposed this research is obtain reduced of matter for
biodegradation lignin in Biological pretreatment with White Rot
Fungi eg. Phanerochaete Chrysosporium using Solid state
Fermentation methods. Phanerochaete Chrysosporium is known to
have the best ability to degraded lignin, but simultaneously it can also
degraded cellulose and hemicelulose. During 8 weeks incubation,
water hyacinth occurred loss of weight reached 34,67%, while loss
of lignin reached 67,21%, loss of cellulose reached 11,01% and loss
of hemicellulose reached 36,56%. The kinetic of losses lignin using
regression linear plot, the results is obtained constant rate (k) of
reduction lignin is -0.1053 and the equation of reduction of lignin
is y = wo - 0, 1.53 x
Biodegradation, lignin, PhanerochaeteChrysosporium, SSF, Water Hyacinth, Bioethanol
Degree of Hydrolysis of Proteinaceous Components of Porang Flour Using Papain
Glucomannan can be found in the tuber of porang together with starch and proteinaceous components which were regarded as impurities. An enzymatic process for obtaining higher glucomannan content from Porang flour have been conducted. Papain was used for hydrolysing proteinaceous components in Porang flour which was conducted after a simultaneous extraction of glucomannan and enzymatic starch hydrolysis. Three variables affecting the rate were studied, i.e. temperature, the amount of enzyme and the stirring speed. The ninhydrin method was used to determine degree of protein hydrolysis. Results showed that the rising of degree of hydrolysis were fast in the first ten minutes of the reaction and then proceeded slowly afterward. The optimum temperature for hydrolysis was 60 oC. Increasing the amount of enzyme showed a remarkable effect to degree of hydrolysis, but the stirring speed had no significant effect. This indicated that the reaction controlled the rate of hydrolysis.
Degree of hydrolysis, ninhydrin, papain, porang flour, proteinaceous components.