Open Science Research Excellence
%0 Journal Article
%A Homayon Ahmad Panahi and  Hossein Sid Kalal and  Atyeh Rahimi
%D 2008 
%J  International Journal of Chemical, Molecular, Nuclear, Materials and Metallurgical Engineering
%B World Academy of Science, Engineering and Technology
%I International Science Index 18, 2008
%T Separation of Vitamin B2 and B12 byImpregnate HPTLC Plates with Boric Acid
%U http://waset.org/publications/6116
%V 18
%X A high performance thin layer chromatography
system (HPTLC) for the separation of vitamin B2 and B12 has been
developed. The separation was successfully using a solvent system of
methanol, water, ammonia 7.3.1 (V/V) as mobile phase on HPTLC
plates impregnated with boric acid. The effect of other mobile phases
on the separation of vitamins was also examined. The method is
based on different behavior of investigated compounds in
impregnated TLC plates with different amount of boric acid. The Rf
values of vitamin B2 and B12 are considered on non impregnated
and impregnated silica gel HPTLC plate with boric acid. The effect
of boric acid in the mobile phase and on HPTLC plates on the RF
values of the vitamins has also been studied.
%P 67 - 69