Open Science Research Excellence

Open Science Index

Commenced in January 2007 Frequency: Monthly Edition: International Publications Count: 29858


Select areas to restrict search in scientific publication database:
7928
High Performance Liquid Chromatography Determination of Urinary Hippuric Acid and Benzoic Acid as Indices for Glue Sniffer Urine
Abstract:
A simple method for the simultaneous determination of hippuric acid and benzoic acid in urine using reversed-phase high performance liquid chromatography was described. Chromatography was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a mobile phase of mixed solution methanol: water: acetic acid (20:80:0.2) and UV detection at 254 nm. The calibration curve was linear within concentration range at 0.125 to 6.0 mg/ml of hippuric acid and benzoic acid. The recovery, accuracy and coefficient variance of hippuric acid were 104.54%, 0.2% and 0.2% respectively and for benzoic acid were 98.48%, 1.25% and 0.60% respectively. The detection limit of this method was 0.01ng/l for hippuric acid and 0.06ng/l for benzoic acid. This method has been applied to the analysis of urine samples from the suspected of toluene abuser or glue sniffer among secondary school students at Johor Bahru.
Digital Object Identifier (DOI):

References:

[1] Serap A.A., Pembe O., Yusuf K., Huseyin K., Suheyla E., "Medicolegal Aspects of Blood-Urine Toluene and Urinary Ortho-Cresol concentration in Toluene Exposure". Journal of Medical Science. 31: 415-419, 2001.
[2] Yacob A.R, Zinalibdin M.R. "Characterization using instrumentation of local glue related glue sniffing activity". Indian Journal of Forensic Medicine and Toxicology. 3:2.58-64, 2009.
[3] Levine B. (2006). "Principles of Forensic Toxicology".(2nd Edition). Part III, Analyte, Inhalants. (pp. 373-380). AACC Press.
[4] Anderson C.E, Loomis G.A. "Recognition and Prevention of Inhalant Abuse". American Academy of Family Physician. 68: 869-874, 2003.
[5] Nicholas .K, Zili S., Maria De La Rosa. "Epidemiology of Inhalant Abuse: An International Perspective". U.S Department of Health and Human Service, Public Health Service, National Institute of Health. Journal of Inhalant abuse. 2-3, 1995.
[6] Inhalant - Wikipedia, the free encyclopedia.htm "Electrochemical behaviour of electrodeposited Ni-WC composite coatings". Surface & Coatings Technology. Article in press.
[7] Yamazaki K, Tanaka E, Misawa S. "Urinary ortho-cresol concentrations as an indicator of toluene inhalation in glue sniffer". Journal of Forensic Science. 32: 215-223, 1992.
[8] Cecilia Z., Diana V.R., Alberto V., Federica V., Bruno G., Massimo C., Irene M. "Determination of urinary hippuric acid by micellar electrokinetic capillary chromatography". Journal of Chromatography B, 793. 223-228, 2003.
[9] International Programme on Chemical Safety (IPCS) (1986). Chemical. Environmental Health Criteria 52: Toluene. WHO. Geneva.
[10] Manabu Y, Atsushi A, Tomoaki M, Toshimitsu W. "Simple Colorimetric semiquantitation of hippuric acid in urine for demonstration of toluene abuse". Journal of Forensic and Legal Medicine, 7: 198-200, 2005.
[11] Yacob A.R., Zinalibdin M.R. "Spectrophotometry Semiquantitation Method Hippuric Acid in Urine for Demonstration of Glue abuser". Indian Journal of Forensic Medicine and Toxicology. In Press 2009.
[12] Kiyoshi K, Yukio H, Keiko K, Takashi I. "Determination of Benzoic Acid and Hippuric Acid in human plasma and urine by High Performance Liquid Chromatography". Journal of Chromatography, 425: 67-75, 1988.
[13] Caret R.L, Denniston K.J, Topping J.J. (1997). "Principles and applications of inorganic, organic and biological chemistry". (2nd Edition). Wm.C. Brown Publisher.
Vol:13 No:07 2019Vol:13 No:06 2019Vol:13 No:05 2019Vol:13 No:04 2019Vol:13 No:03 2019Vol:13 No:02 2019Vol:13 No:01 2019
Vol:12 No:12 2018Vol:12 No:11 2018Vol:12 No:10 2018Vol:12 No:09 2018Vol:12 No:08 2018Vol:12 No:07 2018Vol:12 No:06 2018Vol:12 No:05 2018Vol:12 No:04 2018Vol:12 No:03 2018Vol:12 No:02 2018Vol:12 No:01 2018
Vol:11 No:12 2017Vol:11 No:11 2017Vol:11 No:10 2017Vol:11 No:09 2017Vol:11 No:08 2017Vol:11 No:07 2017Vol:11 No:06 2017Vol:11 No:05 2017Vol:11 No:04 2017Vol:11 No:03 2017Vol:11 No:02 2017Vol:11 No:01 2017
Vol:10 No:12 2016Vol:10 No:11 2016Vol:10 No:10 2016Vol:10 No:09 2016Vol:10 No:08 2016Vol:10 No:07 2016Vol:10 No:06 2016Vol:10 No:05 2016Vol:10 No:04 2016Vol:10 No:03 2016Vol:10 No:02 2016Vol:10 No:01 2016
Vol:9 No:12 2015Vol:9 No:11 2015Vol:9 No:10 2015Vol:9 No:09 2015Vol:9 No:08 2015Vol:9 No:07 2015Vol:9 No:06 2015Vol:9 No:05 2015Vol:9 No:04 2015Vol:9 No:03 2015Vol:9 No:02 2015Vol:9 No:01 2015
Vol:8 No:12 2014Vol:8 No:11 2014Vol:8 No:10 2014Vol:8 No:09 2014Vol:8 No:08 2014Vol:8 No:07 2014Vol:8 No:06 2014Vol:8 No:05 2014Vol:8 No:04 2014Vol:8 No:03 2014Vol:8 No:02 2014Vol:8 No:01 2014
Vol:7 No:12 2013Vol:7 No:11 2013Vol:7 No:10 2013Vol:7 No:09 2013Vol:7 No:08 2013Vol:7 No:07 2013Vol:7 No:06 2013Vol:7 No:05 2013Vol:7 No:04 2013Vol:7 No:03 2013Vol:7 No:02 2013Vol:7 No:01 2013
Vol:6 No:12 2012Vol:6 No:11 2012Vol:6 No:10 2012Vol:6 No:09 2012Vol:6 No:08 2012Vol:6 No:07 2012Vol:6 No:06 2012Vol:6 No:05 2012Vol:6 No:04 2012Vol:6 No:03 2012Vol:6 No:02 2012Vol:6 No:01 2012
Vol:5 No:12 2011Vol:5 No:11 2011Vol:5 No:10 2011Vol:5 No:09 2011Vol:5 No:08 2011Vol:5 No:07 2011Vol:5 No:06 2011Vol:5 No:05 2011Vol:5 No:04 2011Vol:5 No:03 2011Vol:5 No:02 2011Vol:5 No:01 2011
Vol:4 No:12 2010Vol:4 No:11 2010Vol:4 No:10 2010Vol:4 No:09 2010Vol:4 No:08 2010Vol:4 No:07 2010Vol:4 No:06 2010Vol:4 No:05 2010Vol:4 No:04 2010Vol:4 No:03 2010Vol:4 No:02 2010Vol:4 No:01 2010
Vol:3 No:12 2009Vol:3 No:11 2009Vol:3 No:10 2009Vol:3 No:09 2009Vol:3 No:08 2009Vol:3 No:07 2009Vol:3 No:06 2009Vol:3 No:05 2009Vol:3 No:04 2009Vol:3 No:03 2009Vol:3 No:02 2009Vol:3 No:01 2009
Vol:2 No:12 2008Vol:2 No:11 2008Vol:2 No:10 2008Vol:2 No:09 2008Vol:2 No:08 2008Vol:2 No:07 2008Vol:2 No:06 2008Vol:2 No:05 2008Vol:2 No:04 2008Vol:2 No:03 2008Vol:2 No:02 2008Vol:2 No:01 2008
Vol:1 No:12 2007Vol:1 No:11 2007Vol:1 No:10 2007Vol:1 No:09 2007Vol:1 No:08 2007Vol:1 No:07 2007Vol:1 No:06 2007Vol:1 No:05 2007Vol:1 No:04 2007Vol:1 No:03 2007Vol:1 No:02 2007Vol:1 No:01 2007